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Determination of mumps virus antigen in clinical studies of early

Nose of Oral and Maxillofacial Surgery, 2001,7 (4): 263 (Continued from page 11) individual differences related to J. The incidence rate is still high domestic mumps,[link widoczny dla zalogowanych], infectious diseases, the disease proportion increases every year, according to Beijing Children's Hospital reported 60 cases of early hospitalization rate of mumps accounted for 0.77% to 9o's has risen to 35.5% 【6J. Statistics inpatient hospital nearly 30 years, accounting for about 33.3% of mumps patients. Therefore urgent to improve laboratory diagnostic techniques. Mumps antibodies by conventional equipment is simple,[link widoczny dla zalogowanych], no pollution, are widely used, but due to the time of sample collection, preservation methods and detection technology and other factors, there are positive rate is low, the report is not timely and other defects. IFA immunofluorescence using traditional techniques, first with an antibody and antigen in the samples, and then FITC secondary antibody staining, observed under fluorescence microscope set, fluorescent antigen-antibody complex place, so as to make the antigen on the samples identification. Our data show a rapid IFA, timeliness: the body of mumps virus infection or after vaccination immune response, produce antibodies to the convalescent serum (14-21 days) reached a peak 7】. Although the complement fixation method for retrospective diagnosis or for epidemiological survey to understand the level of population immunity,[link widoczny dla zalogowanych], but of little significance for early diagnosis. Mumps virus invasion of the body by the nose and upper airway epithelial cells after propagation, causing local inflammation and immune responses, such as lymphocyte infiltration, increased vascular permeability and IgA secretion,[link widoczny dla zalogowanych], the virus entered the blood circulation of local breeding J. Viremia before the above-mentioned parts of the specimens collected by IFA detection of viral antigens to make an early diagnosis for early isolation and early treatment. Our data also showed good sensitivity of IFA; this study showed that 83% of IFA-positive,[link widoczny dla zalogowanych], the detection rate than the complement fixation method has obvious advantages. In addition, IFA is the mouth through the parotid duct (relative to the maxillary second molar buccal mucosa) at the drawn, easy to operate, fast characteristics. The traditional complement fixation method is a common means of diagnosis of mumps, although its high specificity, but there are drawbacks of poor sensitivity; which: (1) improper blood collection time, some specimens were sampled at the first onset, this time not yet produced antibodies ; (2) serum samples of low quality, submission is not timely, properly preserved specimens cold chain and other relevant, thus affecting their sensitivity. The IFA is not the law of factors, in the acute phase, mumps virus continues to discharge from the respiratory tract, so the higher positive rate. The IFA has a prompt, timely, high sensitivity, easy operation, it is worthy of clinical application.

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