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Dołączył: 03 Mar 2011
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 Wysłany: Pią 14:09, 04 Mar 2011 Temat postu: UGG stivali qde sly rzv dpt |
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Gastric vascular endothelial growth factor expression and its significance
Chronic liver disease detected qualitative and quantitative clinical significance of HBV-DNA Qin Yaqin, Wong Chung-Min (School of Medicine Affiliated Hospital of Youjiang epidemiology, Baise 533000) Keywords: Hepatitis B; hepatitis B virus; DNA; polymerase chain reaction CLC: R512.6 Document code: B Article ID :1004-583X (2004) 18-1062-02 China is hepatitis B virus (HBV) infection in high incidence, the crowd of about HBV infection rate was 6O , where HBV carrier rate of about 1O,[link widoczny dla zalogowanych], many studies have shown that hepatitis B virus surface antigen (HBsAg)-negative HBV viral marker-positive people who are still a small number of low level HBV infection. Our previous 113 cases of HBV infection and 34 HBsAg-negative chronic liver disease were qualitative and quantitative HBV-DNA detection and its clinical features,[link widoczny dla zalogowanych], are as follows. 1 Materials and Methods 1.1 Case Selection for the January 2002 to 2003 outpatients and inpatients and 147 cases in which healthy patient (HBV past infection) 113 cases of chronic liver disease (chronic hepatitis and cirrhosis) 34 cases. All of the subjects HBsAg negative, but hepatitis B virus surface antibody (anti-HBs), hepatitis B virus e antibody (~-HBe), hepatitis B virus core antibody (anti-a HBc) at least one positive diagnosis of infectious diseases and parasitic 2000 National sis Conference revised 1.2 Detection Methods All subjects underwent hepatitis virus markers (HBVM), HBV-DNA detection of qualitative and quantitative, including serum HBVM by enzyme-linked immunosorbent assay method for detection, reagents from Zhongshan Biotechnology Limited; serum HBV qualitative and quantitative-DNA by polymerase chain reaction (PCR), kit was purchased from Shanghai Fuxing Medical Technology Development Co., Ltd., in strict accordance with instructions. HBV-DNA levels> 1.0 × 10. Copies / ml was positive. 1.3 The statistical methods used to compare the positive rate of Y test, measurement data were compared with F test. 2 results of different groups of HBV-DNA 2.1 qualitative and quantitative detection of HBV in chronic liver disease and past infection of qualitative and quantitative HBV-DNA positive rate accounted for 11 (16/147), 14 (21/147), quantitative positive rate The positive rate slightly higher than the qualitative, but the chronic liver disease of qualitative and quantitative HBV-DNA positive rate of previous HBV infection were compared with significantly (Pd0.01), Table 1. Table 1, different groups of people IIBVI) NA qualitative and 2.2 ■ detection of different HBV-DNA HBVM mode detection of qualitative and quantitative HBVM mode 3 there is a certain percentage of HBV-DNA positive rate, where one anti-HBe group, anti-HBc + anti-a A HBe group,[link widoczny dla zalogowanych], three antibody-positive group were not significantly different (P> O.05), slightly higher than the positive rate in each group qualitative quantitative positive rate, in Table 2. Table 2 ItBVM mode ItBV-DNA model of qualitative and quantitative analysis of the number of cases HBVM Note: 3 groups no significant difference between the carriers and chronic liver disease 2.3HBV HBV-DNA copies of index HBV carriers, chronic hepatitis,[link widoczny dla zalogowanych], hepatitis HBV-DNA copy of the cirrhosis group index showed a low level, chronic hepatitis and liver cirrhosis group, HBV carriers with high mean differences between groups was not significant, Table 3. Table 3ItBV carriers and chronic liver disease ItBV-DNA copy of the index Note: The 3 groups showed no significant difference between the 3 discussion of HBV-DNA HBV infection and replication is the most direct indicators, many studies have shown that HBsAg-negative but serum HBV in other marker-positive population still possible infection by HBV, which is an important reason is due to genetic mutation caused s HBsAg was negative in some patients], but there is still a low level in vivo HBV, the main basis for diagnosis in some patients PCR,[link widoczny dla zalogowanych], serum or liver tissue HBV-DNA, serum HBsAg Jiaqiu Long L2 reported negative chronic hepatitis HBV-DNA positive rate was 16.7, Qinghua and other business reported 43 cases of ridicule of unknown cause in patients with chronic viral hepatitis, liver HBV -DNA quantification were 20.9,34.9 positive. Chronic liver disease in this study qualitative and quantitative HBV-DNA positive rates were 26.5,38.2, similar to the above report, HBV past infection also found a few people who were HBV-DNA positive
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